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flow cytometry and the home aquarium
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11-21-2007, 02:50 AM
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tsinkoy
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Quote:
Originally Posted by
Spracklcat
How do you plan on correlating the cytometry reulsts ("there are X different species of organism") to the actual identifications of the organisms?
There is a lot of flow cytometry data on phytoplankton, since these organisms were originally discovered using the technology. Right now, I'm trying to acquire 0.98 um beads to use as a control. The sizes of the most abundant phytoplankton are around that. It's a control bead that we don't normally use in the lab, so I'm trying to see if anyone else has it.
Also, these phytoplankton have fluorescent signatures that are well characterized.
I hope that answers your questions Spracklcat. :-)
I haven't gotten anywhere on this yet, because my boss is away on vacation, and I'm running the facility by my lonesome.
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75G FOWLR
50lbs LR
20G sump/refugium
Quiet One 3000 return pump
Hurricane Quietflo-600 Overflow
2 Maxijet 1200 for flow
Coralife Super Skimmer 220
Coralife 10K T5 lights with Actinics
Livestock:
2 False Percula (
Amphiprion ocellaris
)
1 Emerald crab
1 Kenya Tree (
Capnella sp
)
1 feather duster (
Sabellastarte sp
)
1 colony hairy mushrooms (
Actinodiscus sp
)
numerous hermit crabs
3 turbo snails
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