Just a guess, but after a certain amount of time, I would think that the creatures in your substrate would not necessarily even out, but would end up achieving a balance based on which creatures were more adaptable to a fixed environment such as your tank. The biodiversity, I would think, would increase to a point, plateau, and then would drop off as some species outcompeted others. For example, in my substrate (I don't have a DSB and have been thinking of adding a few scoops of live sand to diversify, by the way, who's got good stuff out there?), the presence of some creatures I used to see has gone down, and now the most visible (very visible, it looks like a carpet) inhabitants are spaghetti worms. There are still numerous copepods and amphipods present in the tank, bristleworms, etc. as well, but it seems like the spaghetti worms have established themselves as the most dominant presence in the substrate. In this respect (if my conjecture is valid) I would think that the addition of new live sand would be a bonus on a periodic, but consistent basis.