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REEFRON

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I've just added about 1/2" of "Oolitic" sand to my existing 1" sandbed. My 110g tank is about a year old and has about 175lbs of LR lots of corals and couple fish. I want to get to about 4", but how much should I add at a time without killing my existing sand critters.
How long should I wait before adding more?
 

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