As far as skimmers go, freshwater doesn't "froth" as well as saltwater, so they won't work as well. Bob Goemens once mentioned something about adding "frothing agents" to freshwater to aid in this, but that's all I know.
As far as DSB, bear in mind different freshwater habitats have different pHs, so for instance aragonite may not be the right choice... In principle, though, a DSB should do well with denitification, just like they do in saltwater, although the lack of sand-stirring & sand-boring creatures may be an issue.
[ September 17, 2001: Message edited by: Cheese Sandwich ]