In my own opinion, you're pushing it if you expect to ship an animal safely with
out using O2 (species-dependent, of course) for longer than 8-10 hours, and I'd only try it on really small, rather inactive fish.
With fishes like tangs and porkys, you are obliged to take those thick bags, fold a piece of newsprint to create an envelope of two or more layers, and place this inside one bag, then placing another bag into that to create a bag-paper-bag sandwich. THIS WORKS! I prefer to use it for everything-I think it helps cushion temperature changes (not much, but EVERY bit helps, eh?), and it creates a darker, more secure environment for the fishes. I also think it can help in the presence of many hard corals (prevent banging, you can use bits of styro in between bags for those). Another way to get something similar would be to use pieces of black trashbags (they're chemically inert without any anti-additives).
Be SURE to twist those bags with AS MUCH O2 in them as you can (whoever said it is right, industry standard is about 1/3 total volume being water, although aged, unused water is acceptable/applicable in some applications), then fold over when banding and twist again. Be sure to band damn tight, too. Are these things going in a plane? If so, ensuring the tightest seal is of paramount importance. Do not underestimate the powers of atmospheric pressures (or the lack thereof).
When packing into the box, you want to ensure a few things:
*Don't let the specimens bounce about the box
*Don't let the bags roll around
*Allow sufficient room for expansion when at altitude--this is often done by alternating bags filled w/air loosely sealed and not filled tight
*Pay attention to the weather!
*As much styro as possible, you really do want ALL sides covered with styro, and in as sealed a configuration as possible (some vendors'll ship in a box with styro panels, instead of a formed box--mina don't like those)
Does this help? Oh yeah, don't feed the day before shipping.
